› 3fy1

X-ray diffraction
1.7Å resolution

The Acidic Mammalian Chitinase catalytic domain in complex with methylallosamidin

Released:
DOI: 10.2210/pdb3fy1/pdb
PDB entry 3fy1 coloured by chain, front view.
PDB entry 3fy1 coloured by chain, side view.
PDB entry 3fy1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Triad of polar residues implicated in pH specificity of acidic mammalian chitinase.
Olland AM, Strand J, Presman E, Czerwinski R, Joseph-McCarthy D, Krykbaev R, Schlingmann G, Chopra R, Lin L, Fleming M, Kriz R, Stahl M, Somers W, Fitz L, Mosyak L
Protein Sci 18 569-78 (2009)
PMID: 19241384

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Acidic mammalian chitinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 395 amino acids
Theoretical weight: 44.2 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q9BZP6 (Residues: 22-408; Coverage: 85%)
Gene name: CHIA
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAA, NA1
Carbohydrate polymer
1 bound ligand:
Ligand AMI 2 x AMI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 63.688Å b: 89.287Å c: 126.689Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.194
Expression system: Cricetulus griseus

Quick links

Citations

5 review citations

Production of chitooligosaccharides and their potential applications in medicine.
Aam et al. (2010)
4 more

8 mentions without citation

Triad of polar residues implicated in pH specificity of acidic mammalian chitinase.
Olland et al. (2009)
7 more