› 3g04

X-ray diffraction
2.55Å resolution

Crystal structure of the TSH receptor in complex with a thyroid-stimulating autoantibody

Released:
DOI: 10.2210/pdb3g04/pdb
PDB entry 3g04 coloured by chain, front view.
PDB entry 3g04 coloured by chain, side view.
PDB entry 3g04 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the TSH receptor in complex with a thyroid-stimulating autoantibody.
Sanders J, Chirgadze DY, Sanders P, Baker S, Sullivan A, Bhardwaja A, Bolton J, Reeve M, Nakatake N, Evans M, Richards T, Powell M, Miguel RN, Blundell TL, Furmaniak J, Smith BR
Thyroid 17 395-410 (2007)
PMID: 17542669

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233989 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HUMAN THYROID STIMULATING AUTOANTIBODY M22 LIGHT CHAIN Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 23.04 KDa
Source organism: Homo sapiens
Expression system: hybrid
Structure domains: Immunoglobulins
HUMAN THYROID STIMULATING AUTOANTIBODY M22 HEAVY CHAIN Chain: B
Molecule details ›
Chain: B
Length: 228 amino acids
Theoretical weight: 24.49 KDa
Source organism: Homo sapiens
Expression system: hybrid
Structure domains: Immunoglobulins
Thyrotropin receptor Chain: C
Molecule details ›
Chain: C
Length: 239 amino acids
Theoretical weight: 26.94 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P16473 (Residues: 22-260; Coverage: 32%)
Gene names: LGR3, TSHR
Sequence domains: BspA type Leucine rich repeat region (6 copies)
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

2 bound ligands:
Ligand NAG 6 x NAG
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: I212121
Unit cell:
a: 43.888Å b: 175.784Å c: 205.806Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.245
Expression systems:
  • hybrid
  • Trichoplusia ni

Quick links

Citations

29 review citations

The significance of G protein-coupled receptor crystallography for drug discovery.
Salon et al. (2011)
28 more

28 mentions without citation

Novel insights on thyroid-stimulating hormone receptor signal transduction.
Kleinau et al. (2013)
27 more