› 3g1n

X-ray diffraction
2.6Å resolution

Catalytic domain of the human E3 ubiquitin-protein ligase HUWE1

Released:
DOI: 10.2210/pdb3g1n/pdb
PDB entry 3g1n coloured by chain, front view.
PDB entry 3g1n coloured by chain, side view.
PDB entry 3g1n coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Walker JR, Qiu L, Li Y, Davis T, Tempel W, Weigelt J, Bountra C, Arrowsmith CH, Edwards AM, Botchkarev A, Dhe-Paganon S, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase HUWE1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 45.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z6Z7 (Residues: 4005-4374; Coverage: 9%)
Gene names: HSPC272, HUWE1, KIAA0312, KIAA1578, UREB1
Sequence domains: HECT-domain (ubiquitin-transferase)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 176.53Å b: 72.213Å c: 77.238Å
α: 90° β: 106.94° γ: 90°
R-values:
R R work R free
0.217 0.214 0.279
Expression system: Escherichia coli

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