› 3g2g

X-ray diffraction
2Å resolution

S437Y Mutant of human muscle pyruvate kinase, isoform M2

Released:
DOI: 10.2210/pdb3g2g/pdb
PDB entry 3g2g coloured by chain, front view.
PDB entry 3g2g coloured by chain, side view.
PDB entry 3g2g coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Hong B, Dimov S, Allali-Hassani A, Tempel W, MacKenzie F, Arrowsmith CH, Edwards AM, Bountra c, Weigelt J, Bochkarev A, Vedadi M, Park H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146984 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKM Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 533 amino acids
Theoretical weight: 58.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14618 (Residues: 1-531; Coverage: 100%)
Gene names: OIP3, PK2, PK3, PKM, PKM2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 13 x SO4
Ligand UNX 31 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P212121
Unit cell:
a: 105.546Å b: 137.885Å c: 155.684Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.26
Expression system: Escherichia coli

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