› 3g73

X-ray diffraction
2.21Å resolution

Structure of the FOXM1 DNA binding

Released:
DOI: 10.2210/pdb3g73/pdb
PDB entry 3g73 coloured by chain, front view.
PDB entry 3g73 coloured by chain, side view.
PDB entry 3g73 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the FoxM1 DNA-recognition domain bound to a promoter sequence.
Littler DR, Alvarez-Fernández M, Stein A, Hibbert RG, Heidebrecht T, Aloy P, Medema RH, Perrakis A
Nucleic Acids Res 38 4527-38 (2010)
PMID: 20360045

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116278 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein M1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 16.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08050 (Residues: 222-360; Coverage: 18%)
Gene names: FKHL16, FOXM1, HFH11, MPP2, WIN
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(P*AP*AP*AP*TP*TP*GP*TP*TP*TP*AP*TP*AP*AP*AP*CP*AP*GP*CP*CP*CP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*TP*TP*CP*GP*GP*GP*CP*TP*GP*TP*TP*TP*AP*TP*AP*AP*AP*CP*AP*AP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 63.07Å b: 119.75Å c: 152.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.234
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

The DREAM complex: master coordinator of cell cycle-dependent gene expression.
Sadasivam et al. (2013)
12 more

15 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
14 more