› 3gjf

X-ray diffraction
1.9Å resolution

Rational development of high-affinity T-cell receptor-like antibodies

Released:
DOI: 10.2210/pdb3gjf/pdb
PDB entry 3gjf coloured by chain, front view.
PDB entry 3gjf coloured by chain, side view.
PDB entry 3gjf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Rational development of high-affinity T-cell receptor-like antibodies.
Stewart-Jones G, Wadle A, Hombach A, Shenderov E, Held G, Fischer E, Kleber S, Nuber N, Stenner-Liewen F, Bauer S, McMichael A, Knuth A, Abken H, Hombach AA, Cerundolo V, Jones EY, Renner C
Proc Natl Acad Sci U S A 106 5784-8 (2009)
PMID: 19307587

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-233914 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Cancer/testis antigen 1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P78358 (Residues: 157-164; Coverage: 4%)
Gene names: CTAG, CTAG1, CTAG1A, CTAG1B, ESO1, LAGE2, LAGE2A, LAGE2B
Antibody light chain Chains: K, L
Molecule details ›
Chains: K, L
Length: 212 amino acids
Theoretical weight: 22.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Antibody heavy chain Chains: H, M
Molecule details ›
Chains: H, M
Length: 220 amino acids
Theoretical weight: 23.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 71.601Å b: 111.841Å c: 124.715Å
α: 90° β: 93.49° γ: 90°
R-values:
R R work R free
0.204 0.202 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

29 review citations

The basic principles of chimeric antigen receptor design.
Sadelain et al. (2013)
28 more

9 mentions without citation

Immunoglobulins or Antibodies: IMGT® Bridging Genes, Structures and Functions.
Lefranc et al. (2020)
8 more