› 3gjo

X-ray diffraction
2.5Å resolution

Crystal structure of human EB1 in complex with microtubule Tip localization signal peptide of MACF

Released:
DOI: 10.2210/pdb3gjo/pdb
PDB entry 3gjo coloured by chain, front view.
PDB entry 3gjo coloured by chain, side view.
PDB entry 3gjo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An EB1-binding motif acts as a microtubule tip localization signal.
Honnappa S, Gouveia SM, Weisbrich A, Damberger FF, Bhavesh NS, Jawhari H, Grigoriev I, van Rijssel FJ, Buey RM, Lawera A, Jelesarov I, Winkler FK, Wüthrich K, Akhmanova A, Steinmetz MO
Cell 138 366-76 (2009)
PMID: 19632184

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169810 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Microtubule-associated protein RP/EB family member 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 72 amino acids
Theoretical weight: 8.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15691 (Residues: 191-260; Coverage: 26%)
Gene name: MAPRE1
Sequence domains: EB1-like C-terminal motif
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Dystonin Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 30 amino acids
Theoretical weight: 3.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03001 (Residues: 7541-7570; Coverage: 0%)
Gene names: BP230, BP240, BPAG1, DMH, DST, DT, KIAA0728

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 45.614Å b: 44.896Å c: 74.84Å
α: 90° β: 98.57° γ: 90°
R-values:
R R work R free
0.215 0.213 0.255
Expression system: Escherichia coli

Quick links

Citations

90 review citations

Store-Operated Calcium Channels.
Prakriya et al. (2015)
89 more

14 mentions without citation

Multisite phosphorylation disrupts arginine-glutamate salt bridge networks required for binding of cytoplasmic linker-associated protein 2 (CLASP2) to end-binding protein 1 (EB1).
Kumar et al. (2012)
13 more