› 3gls

X-ray diffraction
2.7Å resolution

Crystal Structure of Human SIRT3

Released:
DOI: 10.2210/pdb3gls/pdb
PDB entry 3gls coloured by chain, front view.
PDB entry 3gls coloured by chain, side view.
PDB entry 3gls coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of human SIRT3 displaying substrate-induced conformational changes.
Jin L, Wei W, Jiang Y, Peng H, Cai J, Mao C, Dai H, Choy W, Bemis JE, Jirousek MR, Milne JC, Westphal CH, Perni RB
J Biol Chem 284 24394-405 (2009)
PMID: 19535340

Function and Biology Details

Reaction catalysed: 
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent protein deacetylase sirtuin-3, mitochondrial Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 285 amino acids
Theoretical weight: 31.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NTG7 (Residues: 118-399; Coverage: 71%)
Gene names: SIR2L3, SIRT3
Sequence domains: Sir2 family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 6 x ZN
Ligand PGE 2 x PGE
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 85.371Å b: 143.935Å c: 91.657Å
α: 90° β: 117.1° γ: 90°
R-values:
R R work R free
0.185 0.182 0.244
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Mammalian sirtuins: biological insights and disease relevance.
Haigis et al. (2010)
40 more

25 mentions without citation

Sirtuin 1 and sirtuin 3: physiological modulators of metabolism.
Nogueiras et al. (2012)
24 more