› 3gmh

X-ray diffraction
3.95Å resolution

Crystal Structure of the Mad2 Dimer

Released:
DOI: 10.2210/pdb3gmh/pdb
PDB entry 3gmh coloured by chain, front view.
PDB entry 3gmh coloured by chain, side view.
PDB entry 3gmh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of an intermediate conformer of the spindle checkpoint protein Mad2.
Hara M, Özkan E, Sun H, Yu H, Luo X
Proc Natl Acad Sci U S A 112 11252-7 (2015)
PMID: 26305957

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitotic spindle assembly checkpoint protein MAD2A Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 207 amino acids
Theoretical weight: 23.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13257 (Residues: 11-205; Coverage: 95%)
Gene names: MAD2, MAD2L1
Sequence domains: HORMA domain
Structure domains: HORMA domain

Ligands and Environments

1 bound ligand:
Ligand SO4 12 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 124.829Å b: 104.918Å c: 157.746Å
α: 90° β: 97.57° γ: 90°
R-values:
R R work R free
0.22 0.218 0.25
Expression system: Escherichia coli

Quick links

Citations

9 review citations

PRC2 is high maintenance.
Yu et al. (2019)
8 more

3 mentions without citation

Lessons from making the Structural Classification of Proteins (SCOP) and their implications for protein structure modelling.
Andreeva A. (2016)
2 more