› 3gn8

X-ray diffraction
2.5Å resolution

X-ray Crystal Structure of AncGR2 in Complex with Dexamethasone

Released:
DOI: 10.2210/pdb3gn8/pdb
PDB entry 3gn8 coloured by chain, front view.
PDB entry 3gn8 coloured by chain, side view.
PDB entry 3gn8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An epistatic ratchet constrains the direction of glucocorticoid receptor evolution.
Bridgham JT, Ortlund EA, Thornton JW
Nature 461 515-9 (2009)
PMID: 19779450

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163586 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glucocorticoid receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chains: C, E
Molecule details ›
Chains: C, E
Length: 21 amino acids
Theoretical weight: 2.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 734-754; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:
Ligand DEX 2 x DEX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P61
Unit cell:
a: 104.218Å b: 104.218Å c: 144.166Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

39 review citations

Evolution of the cancer genome.
Yates et al. (2012)
38 more

11 mentions without citation

Analyzing protein structure and function using ancestral gene reconstruction.
Harms et al. (2010)
10 more