› 3gqc

X-ray diffraction
2.5Å resolution

Structure of human Rev1-DNA-dNTP ternary complex

Released:
DOI: 10.2210/pdb3gqc/pdb
PDB entry 3gqc coloured by chain, front view.
PDB entry 3gqc coloured by chain, side view.
PDB entry 3gqc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human Rev1-DNA-dNTP ternary complex.
Swan MK, Johnson RE, Prakash L, Prakash S, Aggarwal AK
J Mol Biol 390 699-709 (2009)
PMID: 19464298

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116330 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA repair protein REV1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 504 amino acids
Theoretical weight: 55.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBZ9 (Residues: 330-833; Coverage: 40%)
Gene names: REV1, REV1L
Sequence domains:
Structure domains:
5'-D(*AP*TP*CP*CP*TP*CP*CP*CP*CP*TP*AP*(DOC))-3' Chains: E, G, I, K
Molecule details ›
Chains: E, G, I, K
Length: 12 nucleotides
Theoretical weight: 3.5 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*TP*AP*AP*GP*GP*TP*AP*GP*GP*GP*GP*AP*GP*GP*AP*T)-3' Chains: F, H, J, L
Molecule details ›
Chains: F, H, J, L
Length: 16 nucleotides
Theoretical weight: 5.07 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand DCP 4 x DCP
Ligand MG 13 x MG
1 modified residue:
Ligand DOC 4 x DOC

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 56.436Å b: 172.78Å c: 129.192Å
α: 90° β: 90.65° γ: 90°
R-values:
R R work R free
0.219 0.214 0.267
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

DNA polymerases and cancer.
Lange et al. (2011)
5 more

16 mentions without citation

Translesion DNA polymerases.
Goodman et al. (2013)
15 more