› 3grb

X-ray diffraction
1.75Å resolution

Crystal structure of the F87M/L110M mutant of human transthyretin at pH 6.5

Released:
DOI: 10.2210/pdb3grb/pdb
PDB entry 3grb coloured by chain, front view.
PDB entry 3grb coloured by chain, side view.
PDB entry 3grb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Novel Zn2+-binding sites in human transthyretin: implications for amyloidogenesis and retinol-binding protein recognition.
Palmieri Lde C, Lima LM, Freire JB, Bleicher L, Polikarpov I, Almeida FC, Foguel D
J Biol Chem 285 31731-41 (2010)
PMID: 20659897

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transthyretin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02766 (Residues: 21-147; Coverage: 100%)
Gene names: PALB, TTR
Sequence domains: HIUase/Transthyretin family
Structure domains: Transthyretin/hydroxyisourate hydrolase domain

Ligands and Environments

3 bound ligands:
Ligand ZN 12 x ZN
Ligand GOL 7 x GOL
Ligand ACT 6 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P21
Unit cell:
a: 83.8Å b: 62.197Å c: 47.347Å
α: 90° β: 89.99° γ: 90°
R-values:
R R work R free
0.206 0.203 0.26
Expression system: Escherichia coli

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Citations

4 review citations

Hair analysis as a biomonitor for toxicology, disease and health status.
Kempson et al. (2011)
3 more

1 mention without citation

Novel Zn2+-binding sites in human transthyretin: implications for amyloidogenesis and retinol-binding protein recognition.
Palmieri Lde et al. (2010)