› 3gsu

X-ray diffraction
1.8Å resolution

Crystal structure of the binary complex between HLA-A2 and HCMV NLV-M5T peptide variant

Released:
DOI: 10.2210/pdb3gsu/pdb
PDB entry 3gsu coloured by chain, front view.
PDB entry 3gsu coloured by chain, side view.
PDB entry 3gsu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural bases for the affinity-driven selection of a public TCR against a dominant human cytomegalovirus epitope.
Gras S, Saulquin X, Reiser JB, Debeaupuis E, Echasserieau K, Kissenpfennig A, Legoux F, Chouquet A, Le Gorrec M, Machillot P, Neveu B, Thielens N, Malissen B, Bonneville M, Housset D
J Immunol 183 430-7 (2009)
PMID: 19542454

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137748 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
HCMV pp65 fragment 495-503, variant M5T (NLVPTVATV) Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 913 Da

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 54.095Å b: 81.036Å c: 57.728Å
α: 90° β: 114.2° γ: 90°
R-values:
R R work R free
0.206 0.204 0.242
Expression system: Escherichia coli

Quick links

Citations

9 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
8 more

3 mentions without citation

Broad TCR repertoire and diverse structural solutions for recognition of an immunodominant CD8+ T cell epitope.
Song et al. (2017)
2 more