› 3gxn

X-ray diffraction
3.01Å resolution

Crystal structure of apo alpha-galactosidase A at pH 4.5

Released:
DOI: 10.2210/pdb3gxn/pdb
PDB entry 3gxn coloured by chain, front view.
PDB entry 3gxn coloured by chain, side view.
PDB entry 3gxn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Effects of pH and iminosugar pharmacological chaperones on lysosomal glycosidase structure and stability.
Lieberman RL, D'aquino JA, Ringe D, Petsko GA
Biochemistry 48 4816-27 (2009)
PMID: 19374450

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138990 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-galactosidase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 398 amino acids
Theoretical weight: 45.39 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06280 (Residues: 32-429; Coverage: 100%)
Gene name: GLA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P3221
Unit cell:
a: 90.375Å b: 90.375Å c: 217.524Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.236 0.301
Expression system: Cricetulus griseus

Quick links

Citations

22 review citations

Treating lysosomal storage diseases with pharmacological chaperones: from concept to clinics.
Parenti G. (2009)
21 more

2 mentions without citation

Effects of pH and iminosugar pharmacological chaperones on lysosomal glycosidase structure and stability.
Lieberman et al. (2009)
1 more