› 3gyu

X-ray diffraction
2.4Å resolution

Nuclear receptor DAF-12 from parasitic nematode Strongyloides stercoralis in complex with its physiological ligand dafachronic acid delta 7

Released:
DOI: 10.2210/pdb3gyu/pdb
PDB entry 3gyu coloured by chain, front view.
PDB entry 3gyu coloured by chain, side view.
PDB entry 3gyu coloured by chain, top view.
Source organisms:
Primary publication:
Identification of the nuclear receptor DAF-12 as a therapeutic target in parasitic nematodes.
Wang Z, Zhou XE, Motola DL, Gao X, Suino-Powell K, Conneely A, Ogata C, Sharma KK, Auchus RJ, Lok JB, Hawdon JM, Kliewer SA, Xu HE, Mangelsdorf DJ
Proc Natl Acad Sci U S A 106 9138-43 (2009)
PMID: 19497877

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172400 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear hormone receptor of the steroid/thyroid hormone receptors superfamily Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 28.17 KDa
Source organism: Strongyloides stercoralis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9XZJ5 (Residues: 512-753; Coverage: 32%)
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 1 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.5 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15788 (Residues: 1429-1441; Coverage: 1%)
Gene names: BHLHE74, NCOA1, SRC1

Ligands and Environments

1 bound ligand:
Ligand DL7 1 x DL7
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P65
Unit cell:
a: 120.946Å b: 120.946Å c: 40.757Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.208 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

Sterol regulation of metabolism, homeostasis, and development.
Wollam et al. (2011)
18 more

4 mentions without citation

Identification of the nuclear receptor DAF-12 as a therapeutic target in parasitic nematodes.
Wang et al. (2009)
3 more