› 3h3t

X-ray diffraction
2.4Å resolution

Crystal structure of the CERT START domain in complex with HPA-16

Released:
DOI: 10.2210/pdb3h3t/pdb
PDB entry 3h3t coloured by chain, front view.
PDB entry 3h3t coloured by chain, side view.
PDB entry 3h3t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the CERT START domain with inhibitors provide insights into the mechanism of ceramide transfer.
Kudo N, Kumagai K, Matsubara R, Kobayashi S, Hanada K, Wakatsuki S, Kato R
J Mol Biol 396 245-51 (2010)
PMID: 20036255

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ceramide transfer protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 28.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5P4 (Residues: 347-624; Coverage: 40%)
Gene names: CERT, CERT1, COL4A3BP, STARD11
Sequence domains: START domain
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
Ligand 16H 2 x 16H
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 42.34Å b: 74.568Å c: 78.021Å
α: 90° β: 102.84° γ: 90°
R-values:
R R work R free
0.208 0.205 0.264
Expression system: Escherichia coli

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Citations

14 review citations

Sphingolipids and their metabolism in physiology and disease.
Hannun et al. (2018)
13 more