› 3h44

X-ray diffraction
3Å resolution

Crystal Structure of Insulin Degrading Enzyme in Complex with macrophage inflammatory protein 1 alpha

Released:
DOI: 10.2210/pdb3h44/pdb
PDB entry 3h44 coloured by chain, front view.
PDB entry 3h44 coloured by chain, side view.
PDB entry 3h44 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Ren M, Guo Q, Tang WJ

Function and Biology Details

Reaction catalysed: 
Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145187 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin-degrading enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 990 amino acids
Theoretical weight: 114.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14735 (Residues: 42-1019; Coverage: 96%)
Gene name: IDE
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like
C-C motif chemokine 3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 70 amino acids
Theoretical weight: 7.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10147 (Residues: 23-92; Coverage: 100%)
Gene names: CCL3, G0S19-1, MIP1A, SCYA3
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like

Ligands and Environments

2 bound ligands:
Ligand DIO 6 x DIO
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P65
Unit cell:
a: 262.737Å b: 262.737Å c: 90.502Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.237
Expression system: Escherichia coli

Quick links