› 3h8k

X-ray diffraction
1.8Å resolution

Crystal structure of Ube2g2 complxed with the G2BR domain of gp78 at 1.8-A resolution

Released:
DOI: 10.2210/pdb3h8k/pdb
PDB entry 3h8k coloured by chain, front view.
PDB entry 3h8k coloured by chain, side view.
PDB entry 3h8k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric activation of E2-RING finger-mediated ubiquitylation by a structurally defined specific E2-binding region of gp78.
Das R, Mariano J, Tsai YC, Kalathur RC, Kostova Z, Li J, Tarasov SG, McFeeters RL, Altieri AS, Ji X, Byrd RA, Weissman AM
Mol Cell 34 674-85 (2009)
PMID: 19560420

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158052 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-conjugating enzyme E2 G2 Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P60604 (Residues: 2-165; Coverage: 99%)
Gene names: UBC7, UBE2G2
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
E3 ubiquitin-protein ligase AMFR Chain: B
Molecule details ›
Chain: B
Length: 28 amino acids
Theoretical weight: 3.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKV5 (Residues: 573-600; Coverage: 4%)
Gene names: AMFR, RNF45
Sequence domains: E3 gp78 Ube2g2-binding region (G2BR)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 48.92Å b: 60.15Å c: 61.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.193 0.242
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Building ubiquitin chains: E2 enzymes at work.
Ye et al. (2009)
26 more

23 mentions without citation

RING-type E3 ligases: master manipulators of E2 ubiquitin-conjugating enzymes and ubiquitination.
Metzger et al. (2014)
22 more