› 3hb1

X-ray diffraction
2.51Å resolution

Crystal structure of ed-eya2 complexed with Alf3

Released:
DOI: 10.2210/pdb3hb1/pdb
PDB entry 3hb1 coloured by chain, front view.
PDB entry 3hb1 coloured by chain, side view.
PDB entry 3hb1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of ED-Eya2: insight into dual roles as a protein tyrosine phosphatase and a transcription factor.
Jung SK, Jeong DG, Chung SJ, Kim JH, Park BC, Tonks NK, Ryu SE, Kim SJ
FASEB J 24 560-9 (2010)
PMID: 19858093

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eyes absent homolog 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 274 amino acids
Theoretical weight: 31.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00167 (Residues: 269-538; Coverage: 50%)
Gene names: EAB1, EYA2
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand AF3 4 x AF3
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: I4
Unit cell:
a: 183.941Å b: 183.941Å c: 119.976Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.2 0.24
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The Eyes Absent proteins in development and disease.
Tadjuidje et al. (2013)
10 more

2 mentions without citation

Allosteric inhibitors of the Eya2 phosphatase are selective and inhibit Eya2-mediated cell migration.
Krueger et al. (2014)
1 more