› 3hfb

X-ray diffraction
1.92Å resolution

Crystal structure of human tryoptophan hydroxylase type 1 with LP-534193

Released:
DOI: 10.2210/pdb3hfb/pdb
PDB entry 3hfb coloured by chain, front view.
PDB entry 3hfb coloured by chain, side view.
PDB entry 3hfb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of Inhibition of Novel Tryptophan Hydroxylase Inhibitors Revealed by Co-crystal Structures and Kinetic Analysis.
Cianchetta G, Stouch T, Yu W, Shi ZC, Tari LW, Swanson RV, Hunter MJ, Hoffman ID, Liu Q
Curr Chem Genomics 4 19-26 (2010)
PMID: 20556201

Function and Biology Details

Reaction catalysed: 
L-tryptophan + tetrahydrobiopterin + O(2) = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan 5-hydroxylase 1 Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 33.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P17752 (Residues: 104-393; Coverage: 65%)
Gene names: TPH, TPH1, TPRH, TRPH
Sequence domains: Biopterin-dependent aromatic amino acid hydroxylase
Structure domains: Aromatic amino acid hydroxylase

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand ML4 1 x ML4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 47.354Å b: 58.143Å c: 56.625Å
α: 90° β: 96.77° γ: 90°
R-values:
R R work R free
0.218 0.214 0.289

Quick links

Citations

2 review citations

5-Hydroxytryptophan (5-HTP): Natural Occurrence, Analysis, Biosynthesis, Biotechnology, Physiology and Toxicology.
Maffei ME. (2020)
1 more

3 mentions without citation

Mechanism of Inhibition of Novel Tryptophan Hydroxylase Inhibitors Revealed by Co-crystal Structures and Kinetic Analysis.
Cianchetta et al. (2010)
2 more