› 3hhn

X-ray diffraction
2.99Å resolution

Crystal structure of class I ligase ribozyme self-ligation product, in complex with U1A RBD

Released:
DOI: 10.2210/pdb3hhn/pdb
PDB entry 3hhn coloured by chain, front view.
PDB entry 3hhn coloured by chain, side view.
PDB entry 3hhn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the catalytic core of an RNA-polymerase ribozyme.
Shechner DM, Grant RA, Bagby SC, Koldobskaya Y, Piccirilli JA, Bartel DP
Science 326 1271-5 (2009)
PMID: 19965478

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140520 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chains: B, D
Molecule details ›
Chains: B, D
Length: 97 amino acids
Theoretical weight: 11.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09012 (Residues: 2-98; Coverage: 34%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Class I ligase ribozyme, self-ligation product Chains: C, E
Molecule details ›
Chains: C, E
Length: 137 nucleotides
Theoretical weight: 44.17 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 34 x MG
1 modified residue:
Ligand A23 2 x A23

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P1
Unit cell:
a: 59.561Å b: 70.412Å c: 70.906Å
α: 100.29° β: 99.61° γ: 103.89°
R-values:
R R work R free
0.202 0.202 0.234
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

The noncoding RNA revolution-trashing old rules to forge new ones.
Cech et al. (2014)
18 more

11 mentions without citation

Towards XNA nanotechnology: new materials from synthetic genetic polymers.
Pinheiro et al. (2014)
10 more