› 3hih

X-ray diffraction
1.7Å resolution

Structure of human Plk1-PBD with glycerol and sulfate in the phophopeptide binding site

Released:
DOI: 10.2210/pdb3hih/pdb
PDB entry 3hih coloured by chain, front view.
PDB entry 3hih coloured by chain, side view.
PDB entry 3hih coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analyses of minimal phosphopeptides targeting the polo-box domain of polo-like kinase 1.
Yun SM, Moulaei T, Lim D, Bang JK, Park JE, Shenoy SR, Liu F, Kang YH, Liao C, Soung NK, Lee S, Yoon DY, Lim Y, Lee DH, Otaka A, Appella E, McMahon JB, Nicklaus MC, Burke TR, Yaffe MB, Wlodawer A, Lee KS
Nat Struct Mol Biol 16 876-82 (2009)
PMID: 19597481

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156752 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PLK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 25.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53350 (Residues: 371-593; Coverage: 37%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: POLO box domain

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 5 x GOL
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 33.3Å b: 102.3Å c: 68.5Å
α: 90° β: 93.2° γ: 90°
R-values:
R R work R free
0.188 0.187 0.219
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Polo-box domain: a versatile mediator of polo-like kinase function.
Park et al. (2010)
16 more

4 mentions without citation

Structural and functional analyses of minimal phosphopeptides targeting the polo-box domain of polo-like kinase 1.
Yun et al. (2009)
3 more