› 3hn3

X-ray diffraction
1.7Å resolution

Human beta-glucuronidase at 1.7 A resolution

Released:
DOI: 10.2210/pdb3hn3/pdb
PDB entry 3hn3 coloured by chain, front view.
PDB entry 3hn3 coloured by chain, side view.
PDB entry 3hn3 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Klei HE, Ghosh K, Anumula R

Function and Biology Details

Reaction catalysed: 
A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Beta-glucuronidase Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 613 amino acids
Theoretical weight: 70.76 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
UniProt:
  • Canonical: P08236 (Residues: 21-633; Coverage: 97%)
Gene name: GUSB
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GUP
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand NAG 4 x NAG
Ligand MRD 3 x MRD
Ligand MPD 2 x MPD
Ligand BMA 1 x BMA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 93.578Å b: 123.115Å c: 266.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.24
Expression system: Mus musculus

Quick links