› 3hof

X-ray diffraction
1.9Å resolution

Structure of macrophage migration inhibitory factor (MIF) with caffeic acid at 1.9A resolution

Released:
DOI: 10.2210/pdb3hof/pdb
PDB entry 3hof coloured by chain, front view.
PDB entry 3hof coloured by chain, side view.
PDB entry 3hof coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Crawley L, Barker J, Cheng RKY, Wood M, Felicetti B

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 123 amino acids
Theoretical weight: 13.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14174 (Residues: 1-115; Coverage: 100%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
Ligand DHC 1 x DHC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 67.669Å b: 67.988Å c: 89.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.2
Expression system: Escherichia coli

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