› 3hop

X-ray diffraction
2.3Å resolution

Structure of the actin-binding domain of human filamin A

Released:
DOI: 10.2210/pdb3hop/pdb
PDB entry 3hop coloured by chain, front view.
PDB entry 3hop coloured by chain, side view.
PDB entry 3hop coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Skeletal dysplasias due to filamin A mutations result from a gain-of-function mechanism distinct from allelic neurological disorders.
Clark AR, Sawyer GM, Robertson SP, Sutherland-Smith AJ
Hum Mol Genet 18 4791-800 (2009)
PMID: 19773341

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Filamin-A Chains: A, B
Molecule details ›
Chains: A, B
Length: 272 amino acids
Theoretical weight: 30.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21333 (Residues: 2-269; Coverage: 10%)
Gene names: FLN, FLN1, FLNA
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 57.85Å b: 71.22Å c: 158.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.25 0.288
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Filamins in mechanosensing and signaling.
Razinia et al. (2012)
8 more

4 mentions without citation

ASB2α, an E3 ubiquitin ligase specificity subunit, regulates cell spreading and triggers proteasomal degradation of filamins by targeting the filamin calponin homology 1 domain.
Razinia et al. (2013)
3 more