› 3hqi

X-ray diffraction
2.62Å resolution

Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPMATHx/BTB/3-box-PucSBC1

Released:
DOI: 10.2210/pdb3hqi/pdb
PDB entry 3hqi coloured by chain, front view.
PDB entry 3hqi coloured by chain, side view.
PDB entry 3hqi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases.
Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, White KP, Schulman BA
Mol Cell 36 39-50 (2009)
PMID: 19818708

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129125 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Speckle-type POZ protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 35.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43791 (Residues: 28-329; Coverage: 81%)
Gene name: SPOP
Sequence domains:
Structure domains:
PucSBC1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 amino acids
Theoretical weight: 752 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 57.23Å b: 106.87Å c: 130.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.275
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

67 review citations

Molecular mechanisms of the Keap1–Nrf2 pathway in stress response and cancer evolution.
Taguchi et al. (2011)
66 more

15 mentions without citation

The ubiquitin ligase adaptor SPOP in cancer.
Cuneo et al. (2019)
14 more