› 3htm

X-ray diffraction
2.5Å resolution

Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPBTB/3-box

Released:
DOI: 10.2210/pdb3htm/pdb
PDB entry 3htm coloured by chain, front view.
PDB entry 3htm coloured by chain, side view.
PDB entry 3htm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases.
Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, White KP, Schulman BA
Mol Cell 36 39-50 (2009)
PMID: 19818708

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129123 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Speckle-type POZ protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 172 amino acids
Theoretical weight: 19.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43791 (Residues: 172-329; Coverage: 42%)
Gene name: SPOP
Sequence domains: BTB/POZ domain
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P1
Unit cell:
a: 36.8Å b: 88.7Å c: 88.7Å
α: 90.8° β: 89.3° γ: 89.9°
R-values:
R R work R free
0.217 0.217 0.256
Expression system: Escherichia coli

Quick links

Citations

68 review citations

Molecular mechanisms of the Keap1–Nrf2 pathway in stress response and cancer evolution.
Taguchi et al. (2011)
67 more

5 mentions without citation

Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases.
Zhuang et al. (2009)
4 more