› 3hxi

X-ray diffraction
1.8Å resolution

Crystal structure of Schistosome eIF4E complexed with m7GpppG and 4E-BP

Released:
DOI: 10.2210/pdb3hxi/pdb
PDB entry 3hxi coloured by chain, front view.
PDB entry 3hxi coloured by chain, side view.
PDB entry 3hxi coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into parasite eIF4E binding specificity for m7G and m2,2,7G mRNA caps.
Liu W, Zhao R, McFarland C, Kieft J, Niedzwiecka A, Jankowska-Anyszka M, Stepinski J, Darzynkiewicz E, Jones DN, Davis RE
J Biol Chem 284 31336-49 (2009)
PMID: 19710013

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163644 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic Translation Initiation 4E Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 22.24 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
Eukaryotic translation initiation factor 4E-binding protein 1 Chain: C
Molecule details ›
Chain: C
Length: 21 amino acids
Theoretical weight: 2.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13541 (Residues: 51-67; Coverage: 14%)
Gene name: EIF4EBP1

Ligands and Environments

1 bound ligand:
Ligand GTG 1 x GTG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21212
Unit cell:
a: 45.311Å b: 125.33Å c: 37.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.218 0.272
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Survey of the year 2009: applications of isothermal titration calorimetry.
Falconer et al. (2011)
21 other articles

4 mentions without citation

LARP1 on TOP of ribosome production.
Fonseca et al. (2018)
3 more