› 3i33

X-ray diffraction
1.3Å resolution

Crystal structure of the human 70kDa heat shock protein 2 (Hsp70-2) ATPase domain in complex with ADP and inorganic phosphate

Released:
DOI: 10.2210/pdb3i33/pdb
PDB entry 3i33 coloured by chain, front view.
PDB entry 3i33 coloured by chain, side view.
PDB entry 3i33 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the ATPase domains of four human Hsp70 isoforms: HSPA1L/Hsp70-hom, HSPA2/Hsp70-2, HSPA6/Hsp70B', and HSPA5/BiP/GRP78.
Wisniewska M, Karlberg T, Lehtiö L, Johansson I, Kotenyova T, Moche M, Schüler H
PLoS One 5 e8625 (2010)
PMID: 20072699

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock-related 70 kDa protein 2 Chain: A
Molecule details ›
Chain: A
Length: 404 amino acids
Theoretical weight: 44.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P54652 (Residues: 6-386; Coverage: 60%)
Gene name: HSPA2
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PO4 1 x PO4
Ligand ADP 1 x ADP
Ligand MG 1 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 48.18Å b: 78.6Å c: 93.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.199
Expression system: Escherichia coli

Quick links

Citations

11 review citations

HSPA8/HSC70 chaperone protein: structure, function, and chemical targeting.
Stricher et al. (2013)
10 more

5 mentions without citation

Crystal structures of the ATPase domains of four human Hsp70 isoforms: HSPA1L/Hsp70-hom, HSPA2/Hsp70-2, HSPA6/Hsp70B', and HSPA5/BiP/GRP78.
Wisniewska et al. (2010)
4 more