› 3i4w

X-ray diffraction
1.35Å resolution

Crystal Structure of the third PDZ domain of PSD-95

Released:
DOI: 10.2210/pdb3i4w/pdb
PDB entry 3i4w coloured by chain, front view.
PDB entry 3i4w coloured by chain, side view.
PDB entry 3i4w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Novel conformational aspects of the third PDZ domain of the neuronal post-synaptic density-95 protein revealed from two 1.4A X-ray structures.
Cámara-Artigas A, Murciano-Calles J, Gavira JA, Cobos ES, Martínez JC
J Struct Biol 170 565-9 (2010)
PMID: 20227506

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Disks large homolog 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 104 amino acids
Theoretical weight: 11.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P78352 (Residues: 302-403; Coverage: 14%)
Gene names: DLG4, PSD95
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

1 bound ligand:
Ligand ACT 6 x ACT
1 modified residue:
Ligand SNN 4 x SNN

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P1
Unit cell:
a: 43.322Å b: 42.152Å c: 47.602Å
α: 90° β: 90° γ: 92.08°
R-values:
R R work R free
0.189 0.187 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Contrasting roles of dynamics in protein allostery: NMR and structural studies of CheY and the third PDZ domain from PSD-95.
Lee AL. (2015)
8 other articles

6 mentions without citation

The Conformational Plasticity Vista of PDZ Domains.
Murciano-Calles J. (2020)
5 more