› 3i69

X-ray diffraction
2.38Å resolution

Apo Glutathione Transferase A1-1 GIMF-helix mutant

Released:
DOI: 10.2210/pdb3i69/pdb
PDB entry 3i69 coloured by chain, front view.
PDB entry 3i69 coloured by chain, side view.
PDB entry 3i69 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of a glutathione transferase A1-1 mutant tailored for high catalytic efficiency with toxic alkenals.
Balogh LM, Le Trong I, Kripps KA, Tars K, Stenkamp RE, Mannervik B, Atkins WM
Biochemistry 48 7698-704 (2009)
PMID: 19618965

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase A1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 222 amino acids
Theoretical weight: 25.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08263 (Residues: 1-222; Coverage: 100%)
Gene name: GSTA1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 96.896Å b: 114.684Å c: 97.538Å
α: 90° β: 117.76° γ: 90°
R-values:
R R work R free
0.228 0.225 0.289
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The quest for molecular quasi-species in ligand-activity space and its application to directed enzyme evolution.
Mannervik et al. (2010)
2 more

3 mentions without citation

Interactions of glutathione transferases with 4-hydroxynonenal.
Balogh et al. (2011)
2 more