› 3if8

X-ray diffraction
2.55Å resolution

Crystal Structure of ZWILCH, a member of the RZZ kinetochore complex

Released:
DOI: 10.2210/pdb3if8/pdb
PDB entry 3if8 coloured by chain, front view.
PDB entry 3if8 coloured by chain, side view.
PDB entry 3if8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the RZZ complex reveals common ancestry with multisubunit vesicle tethering machinery.
Civril F, Wehenkel A, Giorgi FM, Santaguida S, Di Fonzo A, Grigorean G, Ciccarelli FD, Musacchio A
Structure 18 616-26 (2010)
PMID: 20462495

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190690 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein zwilch homolog Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 37.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H900 (Residues: 1-334; Coverage: 57%)
Gene name: ZWILCH
Sequence domains: RZZ complex, subunit zwilch
Structure domains:
Protein zwilch homolog Chain: B
Molecule details ›
Chain: B
Length: 257 amino acids
Theoretical weight: 29.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H900 (Residues: 335-591; Coverage: 44%)
Gene name: ZWILCH
Sequence domains: RZZ complex, subunit zwilch
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 86.6Å b: 86.6Å c: 166.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.229 0.274
Expression system: Escherichia coli

Quick links

Citations

11 review citations

A Molecular View of Kinetochore Assembly and Function.
Musacchio et al. (2017)
10 more

5 mentions without citation

Structure of the RZZ complex and molecular basis of its interaction with Spindly.
Mosalaganti et al. (2017)
4 more