› 3ii6

X-ray diffraction
2.4Å resolution

Structure of human Xrcc4 in complex with the tandem BRCT domains of DNA LigaseIV.

Released:
DOI: 10.2210/pdb3ii6/pdb
PDB entry 3ii6 coloured by chain, front view.
PDB entry 3ii6 coloured by chain, side view.
PDB entry 3ii6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional interaction between the human DNA repair proteins DNA ligase IV and XRCC4.
Wu PY, Frit P, Meesala S, Dauvillier S, Modesti M, Andres SN, Huang Y, Sekiguchi J, Calsou P, Salles B, Junop MS
Mol Cell Biol 29 3163-72 (2009)
PMID: 19332554

Function and Biology Details

Reaction catalysed: 
(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156092 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA repair protein XRCC4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 203 amino acids
Theoretical weight: 23.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13426 (Residues: 1-203; Coverage: 60%)
Gene name: XRCC4
Sequence domains: DNA double-strand break repair and V(D)J recombination protein XRCC4
Structure domains:
DNA ligase 4 Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 263 amino acids
Theoretical weight: 30.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49917 (Residues: 654-911; Coverage: 28%)
Gene name: LIG4
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P1
Unit cell:
a: 67.243Å b: 85.982Å c: 111.608Å
α: 67.34° β: 82.86° γ: 74.52°
R-values:
R R work R free
0.24 0.24 0.28
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Detection and repair of ionizing radiation-induced DNA double strand breaks: new developments in nonhomologous end joining.
Wang et al. (2013)
13 more

27 mentions without citation

Repair of double-strand breaks by end joining.
Chiruvella et al. (2013)
26 more