Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149099 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein Nef
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.7 KDa
Source organism: Simian immunodeficiency virus - cpz
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Negative factor, (F-Protein) or Nef
Structure domains: Nef Regulatory Factor
Length: 143 amino acids
Theoretical weight: 16.7 KDa
Source organism: Simian immunodeficiency virus - cpz
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q5QGG3 (Residues: 95-235; Coverage: 54%)
Sequence domains: Negative factor, (F-Protein) or Nef
Structure domains: Nef Regulatory Factor
T-cell surface glycoprotein CD3 zeta chain
Molecule details ›
Chain: B
Length: 45 amino acids
Theoretical weight: 5.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Immunoreceptor tyrosine-based activation motif
Length: 45 amino acids
Theoretical weight: 5.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P20963 (Residues: 51-93; Coverage: 30%)
Sequence domains: Immunoreceptor tyrosine-based activation motif
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
NSLS BEAMLINE X29A
Spacegroup:
P43212
Expression system: Escherichia coli BL21(DE3)
