› 3iqs

X-ray diffraction
2.3Å resolution

Crystal structure of the anti-viral APOBEC3G catalytic domain

Released:
DOI: 10.2210/pdb3iqs/pdb
PDB entry 3iqs coloured by chain, front view.
PDB entry 3iqs coloured by chain, side view.
PDB entry 3iqs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the anti-viral APOBEC3G catalytic domain and functional implications.
Holden LG, Prochnow C, Chang YP, Bransteitter R, Chelico L, Sen U, Stevens RC, Goodman MF, Chen XS
Nature 456 121-4 (2008)
PMID: 18849968

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190763 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA dC->dU-editing enzyme APOBEC-3G Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 22.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HC16 (Residues: 197-380; Coverage: 48%)
Gene names: APOBEC3G, MDS019
Sequence domains: APOBEC-like C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 83.464Å b: 57.329Å c: 40.578Å
α: 90° β: 96.46° γ: 90°
R-values:
R R work R free
0.251 0.251 0.263
Expression system: Escherichia coli

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Citations

29 review citations

Interactions of host APOBEC3 restriction factors with HIV-1 in vivo: implications for therapeutics.
Albin et al. (2010)
28 more

21 mentions without citation

Multiple APOBEC3 restriction factors for HIV-1 and one Vif to rule them all.
Desimmie et al. (2014)
20 more