› 3ir2

X-ray diffraction
2.25Å resolution

Crystal structure of the APOBEC3G catalytic domain

Released:
DOI: 10.2210/pdb3ir2/pdb
PDB entry 3ir2 coloured by chain, front view.
PDB entry 3ir2 coloured by chain, side view.
PDB entry 3ir2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the APOBEC3G catalytic domain reveals potential oligomerization interfaces.
Shandilya SM, Nalam MN, Nalivaika EA, Gross PJ, Valesano JC, Shindo K, Li M, Munson M, Royer WE, Harjes E, Kono T, Matsuo H, Harris RS, Somasundaran M, Schiffer CA
Structure 18 28-38 (2010)
PMID: 20152150

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190763 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA dC->dU-editing enzyme APOBEC-3G Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 24.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HC16 (Residues: 191-384; Coverage: 51%)
Gene names: APOBEC3G, MDS019
Sequence domains: APOBEC-like C-terminal domain

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand CL 2 x CL
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 68.338Å b: 72.532Å c: 97.433Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.166 0.208
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The structural biology of HIV-1: mechanistic and therapeutic insights.
Engelman et al. (2012)
10 more

51 mentions without citation

The APOBEC Protein Family: United by Structure, Divergent in Function.
Salter et al. (2016)
50 more