3iz0

Electron Microscopy
8.6Å resolution

Human Ndc80 Bonsai Decorated Microtubule

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-127130 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.11 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Kinetochore protein NDC80 homolog; Kinetochore protein Spc25 Chains: C, E
Molecule details ›
Chains: C, E
Length: 315 amino acids
Theoretical weight: 36.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14777 (Residues: 80-286; Coverage: 32%)
  • Canonical: Q9HBM1 (Residues: 118-224; Coverage: 48%)
Gene names: AD024, HEC, HEC1, KNTC2, NDC80, SPBC25, SPC25
Sequence domains:
Kinetochore protein Nuf2; Kinetochore protein Spc24 Chains: D, F
Molecule details ›
Chains: D, F
Length: 250 amino acids
Theoretical weight: 28.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BZD4 (Residues: 1-169; Coverage: 36%)
  • Canonical: Q8NBT2 (Residues: 122-197; Coverage: 39%)
Gene names: CDCA1, NUF2, NUF2R, SPBC24, SPC24
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.6Å
Relevant EMDB volumes: EMD-5223
Expression system: Escherichia coli