› 3j2t

Electron Microscopy
9.5Å resolution

An improved model of the human apoptosome

Released:
DOI: 10.2210/pdb3j2t/pdb
PDB entry 3j2t coloured by chain, front view.
PDB entry 3j2t coloured by chain, side view.
PDB entry 3j2t coloured by chain, top view.
Source organisms:
Primary publication:
Changes in Apaf-1 conformation that drive apoptosome assembly.
Yuan S, Topf M, Reubold TF, Eschenburg S, Akey CW
Biochemistry 52 2319-27 (2013)
PMID: 23521171
Related structures: EMD-5186

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127068 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apoptotic protease-activating factor 1 Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 1263 amino acids
Theoretical weight: 144.1 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O14727 (Residues: 1-1248; Coverage: 100%)
Gene names: APAF1, KIAA0413
Sequence domains:
Cytochrome c Chains: H, I, J, K, L, M, N
Molecule details ›
Chains: H, I, J, K, L, M, N
Length: 104 amino acids
Theoretical weight: 11.6 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P62894 (Residues: 2-105; Coverage: 99%)
Gene names: CYC, CYCS
Sequence domains: Cytochrome c

Ligands and Environments

2 bound ligands:
Ligand ATP 7 x ATP
Ligand HEM 7 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.5Å
Relevant EMDB volumes: EMD-5186
Expression system: Spodoptera frugiperda

Quick links

Citations

11 review citations

Apaf-1: Regulation and function in cell death.
Shakeri et al. (2017)
10 more

12 mentions without citation

New insights into apoptosome structure and function.
Dorstyn et al. (2018)
11 more