› 3j82

Electron Microscopy
7.7Å resolution

Electron cryo-microscopy of DNGR-1 in complex with F-actin

Released:
DOI: 10.2210/pdb3j82/pdb
PDB entry 3j82 coloured by chain, front view.
PDB entry 3j82 coloured by chain, side view.
PDB entry 3j82 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the Complex of F-Actin and DNGR-1, a C-Type Lectin Receptor Involved in Dendritic Cell Cross-Presentation of Dead Cell-Associated Antigens.
Hanč P, Fujii T, Iborra S, Yamada Y, Huotari J, Schulz O, Ahrens S, Kjær S, Way M, Sancho D, Namba K, Reis e Sousa C
Immunity 42 839-849 (2015)
PMID: 25979418
Related structures: EMD-6102

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158077 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
C-type lectin domain family 9 member A Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.97 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8BRU4 (Residues: 108-238; Coverage: 55%)
Gene names: Clec9a, Dngr-1
Sequence domains: Lectin C-type domain
Actin, cytoplasmic 1 Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 374 amino acids
Theoretical weight: 41.66 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60709 (Residues: 2-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand ADP 3 x ADP
1 modified residue:
Ligand HIC 3 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 7.7Å
Relevant EMDB volumes: EMD-6102
Expression system: Homo sapiens

Quick links

Citations

19 review citations

Phagocytosis: An Immunobiologic Process.
Gordon S. (2016)
18 more

2 mentions without citation

Structure of the Complex of F-Actin and DNGR-1, a C-Type Lectin Receptor Involved in Dendritic Cell Cross-Presentation of Dead Cell-Associated Antigens.
Hanč et al. (2015)
1 more