› 3jaa

Electron Microscopy
22Å resolution

HUMAN DNA POLYMERASE ETA in COMPLEX WITH NORMAL DNA AND INCO NUCLEOTIDE (NRM)

Released:
DOI: 10.2210/pdb3jaa/pdb
PDB entry 3jaa coloured by chain, front view.
PDB entry 3jaa coloured by chain, side view.
PDB entry 3jaa coloured by chain, top view.
Source organisms:
Primary publication:
Molecular architecture of the Ub-PCNA/Pol η complex bound to DNA.
Lau WC, Li Y, Zhang Q, Huen MS
Sci Rep 5 15759 (2015)
PMID: 26503230
Related structures: EMD-6339

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116425 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase eta Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 48.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y253 (Residues: 1-432; Coverage: 61%)
Gene names: POLH, RAD30, RAD30A, XPV
Sequence domains:
DNA (5'-D(*T*CP*AP*TP*TP*AP*TP*GP*AP*CP*GP*CP*T)-3 Chain: T
Molecule details ›
Chain: T
Length: 13 nucleotides
Theoretical weight: 3.94 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*AP*GP*CP*GP*TP*CP*AP*T)-3') Chain: P
Molecule details ›
Chain: P
Length: 9 nucleotides
Theoretical weight: 2.73 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand DZ4 2 x DZ4
Ligand MG 2 x MG
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 22Å
Relevant EMDB volumes: EMD-6339
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Forging Ahead through Darkness: PCNA, Still the Principal Conductor at the Replication Fork.
Choe et al. (2017)
5 more

3 mentions without citation

Practical lessons from protein structure prediction.
Ginalski et al. (2005)
2 more