› 3jai

Electron Microscopy
3.65Å resolution

Structure of a mammalian ribosomal termination complex with ABCE1, eRF1(AAQ), and the UGA stop codon

Released:
DOI: 10.2210/pdb3jai/pdb
PDB entry 3jai coloured by chain, front view.
PDB entry 3jai coloured by chain, side view.
PDB entry 3jai coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for stop codon recognition in eukaryotes.
Brown A, Shao S, Murray J, Hegde RS, Ramakrishnan V
Nature 524 493-496 (2015)
PMID: 26245381
Related structures: EMD-3040

Function and Biology Details

Reaction catalysed: 
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 87-mer (preferred)
PDBe Complex ID:
PDB-CPX-242977 (preferred)
Entry contents:
80 distinct polypeptide molecules
7 distinct RNA molecules
Macromolecules (87 distinct):
Large ribosomal subunit protein uL2 Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 26.57 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TT27 (Residues: 2-217; Coverage: 85%)
Gene name: RPL8
Sequence domains:
Large ribosomal subunit protein uL3 Chain: B
Molecule details ›
Chain: B
Length: 394 amino acids
Theoretical weight: 45.12 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TL06 (Residues: 2-395; Coverage: 98%)
Gene name: RPL3
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: C
Molecule details ›
Chain: C
Length: 361 amino acids
Theoretical weight: 40.99 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SVW5 (Residues: 2-360; Coverage: 87%)
Gene name: RPL4
Sequence domains:
Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 292 amino acids
Theoretical weight: 34.01 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SYJ6 (Residues: 3-294; Coverage: 98%)
Sequence domains:
60S ribosomal protein L6 Chain: E
Molecule details ›
Chain: E
Length: 248 amino acids
Theoretical weight: 28.53 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TG04 (Residues: 44-250, 251-276; Coverage: 84%)
Sequence domains:
uL30 Chain: F
Molecule details ›
Chain: F
Length: 225 amino acids
Theoretical weight: 26.66 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein eL8 Chain: G
Molecule details ›
Chain: G
Length: 241 amino acids
Theoretical weight: 27.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1STW0 (Residues: 79-319; Coverage: 76%)
Gene name: RPL7A
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Large ribosomal subunit protein uL6 Chain: H
Molecule details ›
Chain: H
Length: 190 amino acids
Theoretical weight: 21.63 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SWI6 (Residues: 1-190; Coverage: 99%)
Gene name: RPL9
Sequence domains: Ribosomal protein L6
Large ribosomal subunit protein uL16 Chain: I
Molecule details ›
Chain: I
Length: 213 amino acids
Theoretical weight: 24.51 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: B7NZQ2 (Residues: 2-214; Coverage: 100%)
Gene name: RPL10
Sequence domains: Ribosomal protein L16p/L10e
Large ribosomal subunit protein uL5 Chain: J
Molecule details ›
Chain: J
Length: 169 amino acids
Theoretical weight: 19.27 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TUB8 (Residues: 9-177; Coverage: 95%)
Gene name: RPL11
Sequence domains:
eL13 Chain: L
Molecule details ›
Chain: L
Length: 210 amino acids
Theoretical weight: 24.22 KDa
Source organism: Oryctolagus cuniculus
eL14 Chain: M
Molecule details ›
Chain: M
Length: 138 amino acids
Theoretical weight: 16.22 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: U3KNW6 (Residues: 2-139; Coverage: 63%)
Sequence domains: Ribosomal protein L14
Large ribosomal subunit protein eL15 Chain: N
Molecule details ›
Chain: N
Length: 203 amino acids
Theoretical weight: 24.08 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T0C1 (Residues: 2-204; Coverage: 100%)
Gene name: RPL15
Sequence domains: Ribosomal L15
uL13 Chain: O
Molecule details ›
Chain: O
Length: 199 amino acids
Theoretical weight: 23.25 KDa
Source organism: Oryctolagus cuniculus
uL22 Chain: P
Molecule details ›
Chain: P
Length: 153 amino acids
Theoretical weight: 17.76 KDa
Source organism: Oryctolagus cuniculus
eL18 Chain: Q
Molecule details ›
Chain: Q
Length: 187 amino acids
Theoretical weight: 21.46 KDa
Source organism: Oryctolagus cuniculus
eL19 Chain: R
Molecule details ›
Chain: R
Length: 180 amino acids
Theoretical weight: 21.61 KDa
Source organism: Oryctolagus cuniculus
eL20 Chain: S
Molecule details ›
Chain: S
Length: 175 amino acids
Theoretical weight: 20.7 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein eL21 Chain: T
Molecule details ›
Chain: T
Length: 159 amino acids
Theoretical weight: 18.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SHQ2 (Residues: 2-160; Coverage: 99%)
Gene name: RPL21
Sequence domains: Ribosomal protein L21e
eL22 Chain: U
Molecule details ›
Chain: U
Length: 99 amino acids
Theoretical weight: 11.48 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein uL14 Chain: V
Molecule details ›
Chain: V
Length: 131 amino acids
Theoretical weight: 13.97 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T6D1 (Residues: 10-140; Coverage: 94%)
Gene name: RPL23
Sequence domains: Ribosomal protein L14p/L23e
eL24 Chain: W
Molecule details ›
Chain: W
Length: 63 amino acids
Theoretical weight: 7.51 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein uL23 Chain: X
Molecule details ›
Chain: X
Length: 119 amino acids
Theoretical weight: 13.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SE76 (Residues: 38-156; Coverage: 76%)
Gene name: RPL23A
Sequence domains:
Large ribosomal subunit protein uL24 Chain: Y
Molecule details ›
Chain: Y
Length: 134 amino acids
Theoretical weight: 15.89 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SQH0 (Residues: 1-134; Coverage: 92%)
Gene name: RPL26
Sequence domains:
Large ribosomal subunit protein eL27 Chain: Z
Molecule details ›
Chain: Z
Length: 135 amino acids
Theoretical weight: 15.7 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TXF6 (Residues: 2-136; Coverage: 99%)
Gene name: RPL27
Sequence domains:
Large ribosomal subunit protein uL15 Chain: a
Molecule details ›
Chain: a
Length: 147 amino acids
Theoretical weight: 16.49 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SNY0 (Residues: 2-148; Coverage: 99%)
Gene name: RPL27A
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein eL29 Chain: b
Molecule details ›
Chain: b
Length: 75 amino acids
Theoretical weight: 8.71 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SGR6 (Residues: 2-76; Coverage: 31%)
Gene name: RPL29
Sequence domains: Ribosomal L29e protein family
eL30 Chain: c
Molecule details ›
Chain: c
Length: 94 amino acids
Theoretical weight: 10.5 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein eL31 Chain: d
Molecule details ›
Chain: d
Length: 107 amino acids
Theoretical weight: 12.64 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SHG0 (Residues: 18-124; Coverage: 86%)
Gene name: RPL31
Sequence domains: Ribosomal protein L31e
Large ribosomal subunit protein eL32 Chain: e
Molecule details ›
Chain: e
Length: 128 amino acids
Theoretical weight: 15.02 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TUN8 (Residues: 24-151; Coverage: 82%)
Gene name: RPL32
Sequence domains: Ribosomal protein L32
Large ribosomal subunit protein eL33 Chain: f
Molecule details ›
Chain: f
Length: 109 amino acids
Theoretical weight: 12.45 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SF08 (Residues: 2-110; Coverage: 99%)
Gene name: RPL35A
Sequence domains: Ribosomal protein L35Ae
eL34 Chain: g
Molecule details ›
Chain: g
Length: 114 amino acids
Theoretical weight: 12.99 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U945 (Residues: 14-127; Coverage: 88%)
Sequence domains: Ribosomal protein L34e
Large ribosomal subunit protein uL29 Chain: h
Molecule details ›
Chain: h
Length: 122 amino acids
Theoretical weight: 14.44 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SIT5 (Residues: 2-123; Coverage: 99%)
Gene name: RPL35
Sequence domains: Ribosomal L29 protein
Large ribosomal subunit protein eL36 Chain: i
Molecule details ›
Chain: i
Length: 102 amino acids
Theoretical weight: 11.89 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TTQ5 (Residues: 26-97; Coverage: 73%)
Gene name: RPL36
Sequence domains: Ribosomal protein L36e
Large ribosomal subunit protein eL37 Chain: j
Molecule details ›
Chain: j
Length: 86 amino acids
Theoretical weight: 10.09 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: U3KPD5 (Residues: 2-87; Coverage: 89%)
Gene name: RPL37
Sequence domains: Ribosomal protein L37e
eL38 Chain: k
Molecule details ›
Chain: k
Length: 69 amino acids
Theoretical weight: 8.11 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein eL39 Chain: l
Molecule details ›
Chain: l
Length: 50 amino acids
Theoretical weight: 6.3 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SYU7 (Residues: 2-51; Coverage: 98%)
Gene name: RPL39
Sequence domains: Ribosomal L39 protein
eL40 Chain: m
Molecule details ›
Chain: m
Length: 52 amino acids
Theoretical weight: 6.2 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein eS32 Chain: n
Molecule details ›
Chain: n
Length: 23 amino acids
Theoretical weight: 3.21 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: A0A087WNH4 (Residues: 2-24; Coverage: 92%)
Gene name: RPL41
Sequence domains: Ribosomal protein L41
Large ribosomal subunit protein eL42 Chain: o
Molecule details ›
Chain: o
Length: 104 amino acids
Theoretical weight: 12.2 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T040 (Residues: 2-100; Coverage: 89%)
Gene name: RPL36A
Sequence domains: Ribosomal protein L44
Large ribosomal subunit protein eL43 Chain: p
Molecule details ›
Chain: p
Length: 91 amino acids
Theoretical weight: 10.17 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SY53 (Residues: 2-92; Coverage: 99%)
Gene name: RPL37A
Sequence domains: Ribosomal L37ae protein family
Large ribosomal subunit protein eL28 Chain: r
Molecule details ›
Chain: r
Length: 125 amino acids
Theoretical weight: 14.32 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U7L1 (Residues: 2-126; Coverage: 91%)
Gene name: RPL28
Sequence domains: Ribosomal L28e protein family
uL10 Chain: s
Molecule details ›
Chain: s
Length: 198 amino acids
Theoretical weight: 21.75 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein uL11 Chain: t
Molecule details ›
Chain: t
Length: 163 amino acids
Theoretical weight: 17.69 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SMR7 (Residues: 1-163; Coverage: 99%)
Gene name: RPL12
Sequence domains:
peptide Chain: 1
Molecule details ›
Chain: 1
Length: 15 amino acids
Theoretical weight: 1.79 KDa
Source organism: Oryctolagus cuniculus
uS2 Chain: AA
Molecule details ›
Chain: AA
Length: 208 amino acids
Theoretical weight: 23.36 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein eS1 Chain: BB
Molecule details ›
Chain: BB
Length: 213 amino acids
Theoretical weight: 24.76 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SS70 (Residues: 21-233; Coverage: 81%)
Gene name: RPS3A
Sequence domains: Ribosomal S3Ae family
uS5 Chain: CC
Molecule details ›
Chain: CC
Length: 218 amino acids
Theoretical weight: 24.05 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TUT9 (Residues: 36-253; Coverage: 81%)
Sequence domains:
Small ribosomal subunit protein uS3 Chain: DD
Molecule details ›
Chain: DD
Length: 227 amino acids
Theoretical weight: 25.16 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TNM3 (Residues: 39-265; Coverage: 81%)
Gene name: RPS3
Sequence domains:
Small ribosomal subunit protein eS4 Chain: EE
Molecule details ›
Chain: EE
Length: 262 amino acids
Theoretical weight: 29.5 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TK17 (Residues: 2-263; Coverage: 100%)
Gene name: RPS4X
Sequence domains:
Small ribosomal subunit protein uS7 Chain: FF
Molecule details ›
Chain: FF
Length: 191 amino acids
Theoretical weight: 21.53 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TFM5 (Residues: 14-204; Coverage: 94%)
Gene name: RPS5
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein eS6 Chain: GG
Molecule details ›
Chain: GG
Length: 237 amino acids
Theoretical weight: 27.47 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TM55 (Residues: 11-211; Coverage: 81%)
Gene name: RPS6
Sequence domains: Ribosomal protein S6e
Small ribosomal subunit protein eS7 Chain: HH
Molecule details ›
Chain: HH
Length: 189 amino acids
Theoretical weight: 21.6 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SVB0 (Residues: 243-431; Coverage: 44%)
Gene name: RPS7
Sequence domains: Ribosomal protein S7e
Small ribosomal subunit protein eS8 Chain: II
Molecule details ›
Chain: II
Length: 206 amino acids
Theoretical weight: 24 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TJW1 (Residues: 2-207; Coverage: 99%)
Gene name: RPS8
Sequence domains: Ribosomal protein S8e
Small ribosomal subunit protein uS4 Chain: JJ
Molecule details ›
Chain: JJ
Length: 185 amino acids
Theoretical weight: 21.65 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: B7NZS8 (Residues: 2-186; Coverage: 95%)
Gene name: RPS9
Sequence domains:
Small ribosomal subunit protein eS10 Chain: KK
Molecule details ›
Chain: KK
Length: 98 amino acids
Theoretical weight: 11.77 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TPV3 (Residues: 1-98; Coverage: 59%)
Sequence domains: Plectin/S10 domain
Small ribosomal subunit protein uS17 Chain: LL
Molecule details ›
Chain: LL
Length: 152 amino acids
Theoretical weight: 17.66 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TRM4 (Residues: 2-153; Coverage: 96%)
Gene name: RPS11
Sequence domains:
Small ribosomal subunit protein eS12 Chain: MM
Molecule details ›
Chain: MM
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SFR8 (Residues: 9-132; Coverage: 94%)
Gene name: RPS12
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Small ribosomal subunit protein uS15 Chain: NN
Molecule details ›
Chain: NN
Length: 150 amino acids
Theoretical weight: 17.13 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SP51 (Residues: 11-152; Coverage: 92%)
Gene name: RPS13
Sequence domains:
Small ribosomal subunit protein uS11 Chain: OO
Molecule details ›
Chain: OO
Length: 136 amino acids
Theoretical weight: 14.54 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T1F0 (Residues: 16-151; Coverage: 90%)
Gene name: RPS14
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS19 Chain: PP
Molecule details ›
Chain: PP
Length: 127 amino acids
Theoretical weight: 15.15 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U0Q2 (Residues: 4-130; Coverage: 88%)
Gene name: RPS15
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein uS9 Chain: QQ
Molecule details ›
Chain: QQ
Length: 141 amino acids
Theoretical weight: 15.98 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SGX4 (Residues: 32-172; Coverage: 82%)
Gene name: RPS16
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein eS17 Chain: RR
Molecule details ›
Chain: RR
Length: 129 amino acids
Theoretical weight: 14.97 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TU13 (Residues: 1-129; Coverage: 96%)
Gene name: RPS17
Sequence domains: Ribosomal S17
uS13 Chain: SS
Molecule details ›
Chain: SS
Length: 137 amino acids
Theoretical weight: 16.17 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SZI5 (Residues: 10-146; Coverage: 88%)
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein eS19 Chain: TT
Molecule details ›
Chain: TT
Length: 141 amino acids
Theoretical weight: 15.68 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TN62 (Residues: 4-144; Coverage: 97%)
Gene name: RPS19
Sequence domains: Ribosomal protein S19e
Small ribosomal subunit protein uS10 Chain: UU
Molecule details ›
Chain: UU
Length: 104 amino acids
Theoretical weight: 11.77 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SIZ2 (Residues: 16-119; Coverage: 87%)
Gene name: RPS20
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein eS21 Chain: VV
Molecule details ›
Chain: VV
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TM82 (Residues: 24-80; Coverage: 51%)
Gene name: RPS21
Sequence domains: Ribosomal protein S21e
Small ribosomal subunit protein uS8 Chain: WW
Molecule details ›
Chain: WW
Length: 129 amino acids
Theoretical weight: 14.73 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TG89 (Residues: 2-130; Coverage: 99%)
Gene name: RPS15A
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS12 Chain: XX
Molecule details ›
Chain: XX
Length: 141 amino acids
Theoretical weight: 15.63 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SZ47 (Residues: 2-142; Coverage: 99%)
Gene name: RPS23
Sequence domains: Ribosomal protein S12/S23
40S ribosomal protein S24 Chain: YY
Molecule details ›
Chain: YY
Length: 126 amino acids
Theoretical weight: 14.6 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T3D8 (Residues: 3-128; Coverage: 96%)
Sequence domains: Ribosomal protein S24e
Small ribosomal subunit protein eS25 Chain: ZZ
Molecule details ›
Chain: ZZ
Length: 75 amino acids
Theoretical weight: 8.53 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TDB3 (Residues: 40-114; Coverage: 61%)
Gene name: RPS25
Sequence domains: S25 ribosomal protein
eS26 Chain: aa
Molecule details ›
Chain: aa
Length: 98 amino acids
Theoretical weight: 11.18 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein eS27 Chain: bb
Molecule details ›
Chain: bb
Length: 83 amino acids
Theoretical weight: 9.35 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TZ76 (Residues: 2-84; Coverage: 99%)
Gene name: RPS27
Sequence domains: Ribosomal protein S27
Small ribosomal subunit protein eS28 Chain: cc
Molecule details ›
Chain: cc
Length: 61 amino acids
Theoretical weight: 6.82 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TIB4 (Residues: 8-68; Coverage: 88%)
Gene name: RPS28
Sequence domains: Ribosomal protein S28e
Small ribosomal subunit protein uS14 Chain: dd
Molecule details ›
Chain: dd
Length: 53 amino acids
Theoretical weight: 6.36 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U7M4 (Residues: 4-56; Coverage: 95%)
Gene name: RPS29
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein eS30 Chain: ee
Molecule details ›
Chain: ee
Length: 57 amino acids
Theoretical weight: 6.51 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T8A2 (Residues: 77-133; Coverage: 43%)
Gene name: FAU
Sequence domains: Ribosomal protein S30
Small ribosomal subunit protein eS31 Chain: ff
Molecule details ›
Chain: ff
Length: 68 amino acids
Theoretical weight: 8 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SK22 (Residues: 115-183; Coverage: 36%)
Gene name: RPS27A
Sequence domains: Ribosomal protein S27a
Small ribosomal subunit protein RACK1 Chain: gg
Molecule details ›
Chain: gg
Length: 313 amino acids
Theoretical weight: 34.67 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SJB4 (Residues: 117-421; Coverage: 72%)
Gene name: RACK1
Sequence domains: WD domain, G-beta repeat
Eukaryotic peptide chain release factor subunit 1 Chain: ii
Molecule details ›
Chain: ii
Length: 416 amino acids
Theoretical weight: 46.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62495 (Residues: 6-421; Coverage: 95%)
Gene names: ERF1, ETF1, RF1, SUP45L1
Sequence domains:
ATP binding cassette subfamily E member 1 Chain: jj
Molecule details ›
Chain: jj
Length: 594 amino acids
Theoretical weight: 66.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SG72 (Residues: 4-597; Coverage: 99%)
Sequence domains:
tRNA(Val) Chain: 2
Molecule details ›
Chain: 2
Length: 76 nucleotides
Theoretical weight: 24.44 KDa
Sequence domains: tRNA
tRNA(Lys) Chain: 3
Molecule details ›
Chain: 3
Length: 75 nucleotides
Theoretical weight: 24.1 KDa
Sequence domains: tRNA
28S ribosomal RNA Chain: 5
Molecule details ›
Chain: 5
Length: 3662 nucleotides
Theoretical weight: 1186.58 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
5S ribosomal RNA Chain: 7
Molecule details ›
Chain: 7
Length: 120 nucleotides
Theoretical weight: 38.69 KDa
Sequence domains: 5S ribosomal RNA
5.8S ribosomal RNA Chain: 8
Molecule details ›
Chain: 8
Length: 156 nucleotides
Theoretical weight: 50.14 KDa
Sequence domains: 5.8S ribosomal RNA
18S Ribosomal RNA Chain: 9
Molecule details ›
Chain: 9
Length: 1719 nucleotides
Theoretical weight: 554.75 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
Messenger RNA Chain: hh
Molecule details ›
Chain: hh
Length: 12 nucleotides
Theoretical weight: 3.84 KDa

Ligands and Environments

4 bound ligands:
Ligand MG 195 x MG
Ligand ZN 8 x ZN
Ligand SF4 2 x SF4
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.65Å
Relevant EMDB volumes: EMD-3040
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

36 review citations

Ribosome-associated protein quality control.
Brandman et al. (2016)
35 more

5 mentions without citation

Decoding Mammalian Ribosome-mRNA States by Translational GTPase Complexes.
Shao et al. (2016)
4 more