› 3jbk

Electron Microscopy
8.2Å resolution

Cryo-EM reconstruction of the metavinculin-actin interface

Released:
DOI: 10.2210/pdb3jbk/pdb
PDB entry 3jbk coloured by chain, front view.
PDB entry 3jbk coloured by chain, side view.
PDB entry 3jbk coloured by chain, top view.
Source organisms:
Primary publication:
The Structural Basis of Actin Organization by Vinculin and Metavinculin.
Kim LY, Thompson PM, Lee HT, Pershad M, Campbell SL, Alushin GM
J Mol Biol 428 10-25 (2016)
PMID: 26493222
Related structures: EMD-6447

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148322 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 41.86 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Vinculin Chain: M
Molecule details ›
Chain: M
Length: 273 amino acids
Theoretical weight: 29.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18206 (Residues: 858-1129; Coverage: 24%)
Gene name: VCL
Sequence domains: Vinculin family

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.2Å
Relevant EMDB volumes: EMD-6447
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Mechanisms and Functions of Vinculin Interactions with Phospholipids at Cell Adhesion Sites.
Izard et al. (2016)
6 more

4 mentions without citation

The Structural Basis of Actin Organization by Vinculin and Metavinculin.
Kim et al. (2016)
3 more