› 3jc1

Electron Microscopy
4Å resolution

Electron cryo-microscopy of the IST1-CHMP1B ESCRT-III copolymer

Released:
DOI: 10.2210/pdb3jc1/pdb
PDB entry 3jc1 coloured by chain, front view.
PDB entry 3jc1 coloured by chain, side view.
PDB entry 3jc1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and membrane remodeling activity of ESCRT-III helical polymers.
McCullough J, Clippinger AK, Talledge N, Skowyra ML, Saunders MG, Naismith TV, Colf LA, Afonine P, Arthur C, Sundquist WI, Hanson PI, Frost A
Science 350 1548-51 (2015)
PMID: 26634441
Related structures: EMD-6461

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 68-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156923 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IST1 homolog Chains: Aa, Ac, Ae, Ag, Ai, Ak, Am, Ao, Aq, As, Au, Aw, Ay, Ba, Bc, Be, Bg, Bi, Bk, Bm, Bo, Bq, Bs, Bu, Bw, By, Ca, Cc, Ce, Cg, Ci, Ck, Cm, Co
Molecule details ›
Chains: Aa, Ac, Ae, Ag, Ai, Ak, Am, Ao, Aq, As, Au, Aw, Ay, Ba, Bc, Be, Bg, Bi, Bk, Bm, Bo, Bq, Bs, Bu, Bw, By, Ca, Cc, Ce, Cg, Ci, Ck, Cm, Co
Length: 182 amino acids
Theoretical weight: 20.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53990 (Residues: 6-187; Coverage: 50%)
Gene names: IST1, KIAA0174
Sequence domains: Regulator of Vps4 activity in the MVB pathway
Charged multivesicular body protein 1b Chains: Ab, Ad, Af, Ah, Aj, Al, An, Ap, Ar, At, Av, Ax, Az, Bb, Bd, Bf, Bh, Bj, Bl, Bn, Bp, Br, Bt, Bv, Bx, Bz, Cb, Cd, Cf, Ch, Cj, Cl, Cn, Cp
Molecule details ›
Chains: Ab, Ad, Af, Ah, Aj, Al, An, Ap, Ar, At, Av, Ax, Az, Bb, Bd, Bf, Bh, Bj, Bl, Bn, Bp, Br, Bt, Bv, Bx, Bz, Cb, Cd, Cf, Ch, Cj, Cl, Cn, Cp
Length: 160 amino acids
Theoretical weight: 17.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7LBR1 (Residues: 4-163; Coverage: 80%)
Gene names: C18orf2, CHMP1B
Sequence domains: Snf7

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-6461
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

47 review citations

The many functions of ESCRTs.
Vietri et al. (2020)
46 more

9 mentions without citation

Practical lessons from protein structure prediction.
Ginalski et al. (2005)
8 more