› 3jsd

X-ray diffraction
2.5Å resolution

Insulin's biosynthesis and activity have opposing structural requirements: a new factor in neonatal diabetes mellitus

Released:
DOI: 10.2210/pdb3jsd/pdb
PDB entry 3jsd coloured by chain, front view.
PDB entry 3jsd coloured by chain, side view.
PDB entry 3jsd coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Weiss MA, Wan ZL, Dodson EJ, Liu M, Xu B, Hua QX, Turkenburg M, Whittingham J, Nakagawa SH, Huang K, Hu SQ, Jia WH, Wang SH, Brange J, Whittaker J, Arvan P, Katsoyannis PG, Dodson GG

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand IPH 1 x IPH
1 modified residue:
Ligand DAL 2 x DAL

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: R3
Unit cell:
a: 80.843Å b: 80.843Å c: 38.842Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.274
Expression system: Not provided

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