› 3jv4

X-ray diffraction
3.15Å resolution

Crystal structure of the dimerization domains p50 and RelB

Released:
DOI: 10.2210/pdb3jv4/pdb
PDB entry 3jv4 coloured by chain, front view.
PDB entry 3jv4 coloured by chain, side view.
PDB entry 3jv4 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A structural basis for selective dimerization by NF-κB RelB.
Vu D, Huang DB, Vemu A, Ghosh G
J Mol Biol 425 1934-1945 (2013)
PMID: 23485337

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150585 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription factor RelB Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 101 amino acids
Theoretical weight: 11.41 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q04863 (Residues: 278-378; Coverage: 18%)
Gene name: Relb
Sequence domains: Rel homology dimerisation domain
Structure domains: Immunoglobulins
Nuclear factor NF-kappa-B p50 subunit Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 115 amino acids
Theoretical weight: 13.39 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P25799 (Residues: 245-359; Coverage: 12%)
Gene name: Nfkb1
Sequence domains: Rel homology dimerisation domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 106.173Å b: 106.173Å c: 143.778Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.28
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

NF-κB pathways in hematological malignancies.
Gasparini et al. (2014)
3 more