› 3jw8

X-ray diffraction
2.1Å resolution

Crystal structure of human mono-glyceride lipase

Released:
DOI: 10.2210/pdb3jw8/pdb
PDB entry 3jw8 coloured by chain, front view.
PDB entry 3jw8 coloured by chain, side view.
PDB entry 3jw8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for human monoglyceride lipase inhibition.
Bertrand T, Augé F, Houtmann J, Rak A, Vallée F, Mikol V, Berne PF, Michot N, Cheuret D, Hoornaert C, Mathieu M
J Mol Biol 396 663-73 (2010)
PMID: 19962385

Function and Biology Details

Reaction catalysed: 
Hydrolyzes glycerol monoesters of long-chain fatty acids
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-189244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monoglyceride lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 320 amino acids
Theoretical weight: 35.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99685 (Residues: 1-303; Coverage: 100%)
Gene name: MGLL
Sequence domains: Serine aminopeptidase, S33
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand MPD 2 x MPD
Ligand MRD 5 x MRD
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I222
Unit cell:
a: 86.588Å b: 126.311Å c: 140.567Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.157 0.189
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

FAT SIGNALS--lipases and lipolysis in lipid metabolism and signaling.
Zechner et al. (2012)
16 more

15 mentions without citation

Monoglyceride lipase: Structure and inhibitors.
Scalvini et al. (2016)
14 more