› 3k27

X-ray diffraction
1.76Å resolution

Complex structure of EED and trimethylated H3K9

Released:
DOI: 10.2210/pdb3k27/pdb
PDB entry 3k27 coloured by chain, front view.
PDB entry 3k27 coloured by chain, side view.
PDB entry 3k27 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Binding of different histone marks differentially regulates the activity and specificity of polycomb repressive complex 2 (PRC2).
Xu C, Bian C, Yang W, Galka M, Ouyang H, Chen C, Qiu W, Liu H, Jones AE, MacKenzie F, Pan P, Li SS, Wang H, Min J
Proc Natl Acad Sci U S A 107 19266-71 (2010)
PMID: 20974918

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131020 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polycomb protein EED Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 42.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75530 (Residues: 76-441; Coverage: 83%)
Gene name: EED
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
HISTONE PEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.02 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 57.961Å b: 85.33Å c: 91.198Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.198
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

52 review citations

Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put.
Simon et al. (2013)
51 more

7 mentions without citation

Readers of histone modifications.
Yun et al. (2011)
6 more