› 3kjo

X-ray diffraction
1.8Å resolution

Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)

Released:
DOI: 10.2210/pdb3kjo/pdb
PDB entry 3kjo coloured by chain, front view.
PDB entry 3kjo coloured by chain, side view.
PDB entry 3kjo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
How telomeric protein POT1 avoids RNA to achieve specificity for single-stranded DNA.
Nandakumar J, Podell ER, Cech TR
Proc Natl Acad Sci U S A 107 651-6 (2010)
PMID: 20080730

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113130 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
Protection of telomeres protein 1 Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 33.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NUX5 (Residues: 1-299; Coverage: 47%)
Gene name: POT1
Sequence domains:
Structure domains: Nucleic acid-binding proteins
DNA/RNA (5'-D(*T)-R(P*U)-D(P*AP*GP*GP*GP*TP*TP*AP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.12 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 101.867Å b: 103.591Å c: 71.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.238
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

15 review citations

The application of DNA and RNA G-quadruplexes to therapeutic medicines.
Collie et al. (2011)
14 more

4 mentions without citation

Unraveling secrets of telomeres: one molecule at a time.
Lin et al. (2014)
3 more