› 3kkt

X-ray diffraction
2.48Å resolution

Crystal structure of human PDE4b with 5-[3-[(1S,2S,4R)-Bicyclo[2.2.1]hept-2-yloxy]-4-methoxyp henyl]tetrahydro-2(1H)-pyrimidinone reveals ordering of the C-terminal helix residues 502-509.

Released:
DOI: 10.2210/pdb3kkt/pdb
PDB entry 3kkt coloured by chain, front view.
PDB entry 3kkt coloured by chain, side view.
PDB entry 3kkt coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Cheng RKY, Crawley L, Barker J, Whittaker M

Function and Biology Details

Reaction catalysed: 
Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-170460 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3',5'-cyclic-AMP phosphodiesterase 4B Chains: A, B
Molecule details ›
Chains: A, B
Length: 398 amino acids
Theoretical weight: 45.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07343 (Residues: 324-700; Coverage: 51%)
Gene names: DPDE4, PDE4B
Sequence domains: 3'5'-cyclic nucleotide phosphodiesterase
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand 0CP 2 x 0CP
Ligand MG 2 x MG
Ligand B3P 1 x B3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P6522
Unit cell:
a: 89.74Å b: 89.74Å c: 337.443Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.235 0.295
Expression system: Escherichia coli

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