› 3kov

X-ray diffraction
2.9Å resolution

Structure of MEF2A bound to DNA reveals a completely folded MADS-box/MEF2 domain that recognizes DNA and recruits transcription co-factors

Released:
DOI: 10.2210/pdb3kov/pdb
PDB entry 3kov coloured by chain, front view.
PDB entry 3kov coloured by chain, side view.
PDB entry 3kov coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the MADS-box/MEF2 domain of MEF2A bound to DNA and its implication for myocardin recruitment.
Wu Y, Dey R, Han A, Jayathilaka N, Philips M, Ye J, Chen L
J Mol Biol 397 520-33 (2010)
PMID: 20132824

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116496 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Myocyte-specific enhancer factor 2A Chains: A, B, I, J
Molecule details ›
Chains: A, B, I, J
Length: 90 amino acids
Theoretical weight: 10.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02078 (Residues: 2-91; Coverage: 18%)
Gene names: MEF2, MEF2A
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
DNA (5'-D(*AP*AP*CP*TP*AP*TP*TP*TP*AP*TP*AP*AP*G)-3') Chains: C, K
Molecule details ›
Chains: C, K
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*CP*TP*TP*AP*TP*AP*AP*AP*TP*AP*GP*T)-3') Chains: D, L
Molecule details ›
Chains: D, L
Length: 13 nucleotides
Theoretical weight: 3.96 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21212
Unit cell:
a: 77.801Å b: 77.96Å c: 106.794Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.221 0.279
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Involvement of myocyte enhancer factor 2c in the pathogenesis of autism spectrum disorder.
Chaudhary et al. (2021)
18 other articles

11 mentions without citation

Structural Basis for Plant MADS Transcription Factor Oligomerization.
Lai et al. (2019)
10 more